Composite

Part:BBa_K2015012:Design

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


LacI + promoter + RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You can amplify this fragment following two primers. 

Forward: GGGGAATTCTTTACAGCTAGCTCAGTCCTAG(starting from EcoRI site)
 
Reverse: CCCACTAGTTTTCTCCTCTTTTCTAGTTGTGC(starting from SpeI site)


Source

gBlocks® Gene Fragments from IDT

References